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On the Cover: Maize root tips die quickly following oxygen deprivation. However, tolerance of anoxia can be greatly improved by brief acclimation under hypoxia (3% oxygen). This acclimation involves protein synthesis. Chang et al. (pp. 295-317) describe the complex patterns of protein synthesis during hypoxic acclimation using the tools of proteomics. Proteins synthesized before, during, and after stress were arrayed by molecular mass and pI on two-dimensional gels. Radiolabel in protein spots was quantified to give relative rates of synthesis, while protein identities were determined by mass spectrometry, matching tryptic fragment masses to gene sequences in databases.
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