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Published on November 2, 2007; 10.1104/pp.107.111575

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Received October 23, 2007
Accepted October 25, 2007

A set of modular binary vectors for the transformation of cereals

Axel Himmelbach , Uwe Zierold , Götz Hensel , Jan Riechen , Dimitar Douchkov , Patrick Schweizer , and Jochen Kumlehn *

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, D-06466 Gatersleben, Germany

* Corresponding author; email: kumlehn{at}ipk-gatersleben.de.

Genetic transformation of crop plants offers the possibility to test hypotheses about the function of individual genes as well as the exploitation of transgenes for targeted trait improvement. However, in most cereals this option has long been compromised by tedious and low efficiency transformation protocols as well as by the lack of versatile vector systems. After having adopted and further improved the protocols for Agrobacterium-mediated stable transformation of barley and wheat, we now present a versatile set of binary vectors for trangene over-expression as well as for gene silencing by dsRNAi. The vector set is offered with a series of functionally validated promoters and allows for rapid integration of the desired genes or gene fragments by GATEWAYTM-based recombination. Additional inbuilt flexibility lies in the choice of plant selectable markers, cassette orientation, and simple integration of further promoters to drive specific expression of genes of interest. Functionality of the cereal vector set has been demonstrated by transient as well as stable transformation experiments for transgene over-expression as well as for targeted gene silencing in barley.






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