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Plant Physiol, December 2000, Vol. 124, pp. 1854-1865
Overexpression of the Arabidopsis CBF3
Transcriptional Activator Mimics Multiple Biochemical Changes
Associated with Cold Acclimation1
Sarah J.
Gilmour,
Audrey M.
Sebolt,
Maite P.
Salazar,
John D.
Everard, and
Michael F.
Thomashow*
Department of Crop and Soil Sciences, Michigan State University,
East Lansing, Michigan 48824-1325 (S.J.G., A.M.S., M.P.S.,
M.F.T.); and Dupont Agricultural Products Biotechnology Experiment
Station 402/5238, Wilmington, Delaware 19880-0402 (J.D.E.)
We further investigated the role of the Arabidopsis
CBF regulatory genes in cold acclimation, the process
whereby certain plants increase in freezing tolerance upon exposure to
low temperature. The CBF genes, which are rapidly
induced in response to low temperature, encode transcriptional
activators that control the expression of genes containing the
C-repeat/dehydration responsive element DNA regulatory element in their
promoters. Constitutive expression of either CBF1 or
CBF3 (also known as DREB1b and
DREB1a, respectively) in transgenic Arabidopsis plants
has been shown to induce the expression of target COR
(cold-regulated) genes and to enhance freezing tolerance in
nonacclimated plants. Here we demonstrate that overexpression of
CBF3 in Arabidopsis also increases the freezing
tolerance of cold-acclimated plants. Moreover, we show that it results
in multiple biochemical changes associated with cold acclimation:
CBF3-expressing plants had elevated levels of proline
(Pro) and total soluble sugars, including sucrose, raffinose, glucose,
and fructose. Plants overexpressing CBF3 also had
elevated P5CS transcript levels suggesting that the
increase in Pro levels resulted, at least in part, from increased
expression of the key Pro biosynthetic enzyme
 1-pyrroline-5-carboxylate synthase. These results lead
us to propose that CBF3 integrates the activation of
multiple components of the cold acclimation response.
1
This work was supported in part by a subcontract
(no. 593-0219-06) under the U.S. Department of
Agriculture/Cooperative State Research, Education and Extension
Service Cooperative Agreement (no. 96-34340-2711) North
Central Biotechnology Initiative and by funds from Mendel
Biotechnology, Inc., and the Michigan Agricultural Experiment Station.
*
Corresponding author; e-mail thomash6{at}pilot.msu.edu; fax
517-353-5174.
© 2000 American Society of Plant Physiologists
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