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Published on July 16, 2008; 10.1104/pp.108.123042

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Received May 15, 2008
Accepted July 9, 2008

Expression of Umbelopsis ramanniana DGAT2A in Seed Increases Oil in Soybean

Kathryn Lardizabal *, Roger Effertz , Charlene Levering , Jennifer Mai , M.C. Pedroso , Tom Jury , Eric Aasen , Ken Gruys , and Kristen Bennett

Calgene Campus, Monsanto Company, 1920 Fifth Street, Davis, CA 95616, USA

* Corresponding author; email: kathy.lardizabal{at}monsanto.com.

Oilseeds are the main source of lipids used in both food and bio-fuels. The growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. Globally, soybean is one of the most important oilseed crops grown contributing about 30% of the vegetable oil used for food, feed and industrial applications. Breeding efforts in soy have shown that multiple loci contribute to the final content of oil and protein stored in seeds. Genetically, the levels of these two storage products appear to be inversely correlated with an increase in oil coming at the expense of protein and vice versa. One way to overcome the linkage between oil and protein is to introduce a transgene that can specifically modulate one pathway without disrupting the other. We describe the first transgenic soy crop with increased oil that shows no major impact on protein content or yield. This was achieved by expressing a codon-optimized version of a diacylglycerol acyltransferase (DGAT2A) from the soil fungus Umbelopsis (formerly Mortierella) ramanniana in soybean seed during development resulting in an absolute increase in oil of 1.5% (by weight) in the mature seed.






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