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Molecular Biology and Gene Regulation

Light and Fungal Elicitor Induce 3-Deoxy-D-arabino-Heptulosonate 7-Phosphate Synthase mRNA in Suspension Cultured Cells of Parsley (Petroselinum crispum L.) ¹

Department of Horticulture, Purdue University, West Lafayette, Indiana 47906, Department of Biochemistry, Purdue University, West Lafayette, Indiana 47906, Department of Biochemistry and Biophysics, University of California, Davis, California 95616

Light and fungal elicitor induce mRNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase in suspension cultured cells of parsley (Petroselinum crispum L.). The kinetics and dose response of mRNA accumulation were similar for DAHP synthase and phenylalanine ammonia-lyase (PAL). Six micrograms of elicitor from Phytophthora megasperma f. glycinia gave a detectable induction within 1 hour. Induction of DAHP synthase and PAL mRNAs by light was transient, reaching maximal levels at 4 hours and returning to pretreatment levels after 24 hours. Our data suggest that either light or fungal elicitor transcriptionally activate DAHP synthase. A coordinate regulation for key enzymes in the synthesis of primary and secondary metabolites is indicated.

¹ This work was supported in part by grants from David Ross (J.M.H.), the McKnight Foundation (K.F.M.) and U.S. Public Health Service GM-05301-29 (E.E.C.).

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