Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 96:993-996 (1991)
© 1991 American Society of Plant Biologists

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Environmental and Stress Physiology

{alpha}-Amylase Inhibitor, Not Phytohemagglutinin, Explains Resistance of Common Bean Seeds to Cowpea Weevil 1

Joseph E. Huesing, Richard E. Shade, Maarten J. Chrispeels and Larry L. Murdock

Department of Entomology, Purdue University, West Lafayette, Indiana 47907-1158, Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0116

There are claims that phytohemagglutinin (PHA), the lectin of common bean, Phaseolus vulgaris, is toxic when fed to the cowpea weevil, Callosobruchus maculatus, and that PHA serves as the chemical defense against this seed-feeding bruchid beetle (DH Janzen, HB Juster, IE Liener [1976] Science 192: 795-796; AMR Gatehouse, FM Dewey, J Dove, KA Fenton, A Pusztai [1984] J Sci Food Agric 35: 373-380). However, our studies indicate that neither PHA nor its isolectins have detrimental effects when fed to the cowpea weevil. To explain these contradictory results we characterized the commercial lectin source used by A. M. R. Gatehouse, F. M. Dewey, J. Dove, K. A. Fenton, A. Pusztai (1984, J Sci Food Agric 35: 373-380). We demonstrate here that the toxic effects of PHA to cowpea weevil are due to an {alpha}-amylase inhibitor contaminant in the commercial preparation.

1 This research was supported by a USAID Title XII Program Support Grant to Purdue University. Purdue University AES Journal Paper 12723.




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