Plant Physiology 96:650-655 (1991)
© 1991 American Society of Plant Biologists
Environmental and Stress Physiology
Response of Photosynthesis and Cellular Antioxidants to Ozone in Populus Leaves 1
Ashima Sen Gupta,
Ruth Grene Alscher and
Delbert McCune
Boyce Thompson Institute, Cornell University, Ithaca, New York 14953,
Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061
Atmospheric ozone causes formation of various highly reactive intermediates (e.g. peroxyl and superoxide radicals, H2O2, etc.) in plant tissues. A plant's productivity in environments with ozone may be related to its ability to scavenge the free radicals formed. The effects of ozone on photosynthesis and some free radical scavengers were measured in the fifth emergent leaf of poplars. Clonal poplars (Populus deltoides x Populus cv caudina) were fumigated with 180 parts per billion ozone for 3 hours. Photosynthesis was measured before, during, and after fumigation. During the first 90 minutes of ozone exposure, photosynthetic rates were unaffected but glutathione levels and superoxide dismutase activity increased. After 90 minutes of ozone exposure, photosynthetic rates began to decline while glutathione and superoxide dismutase continued to increase. Total glutathione (reduced plus oxidized) increased in fumigated leaves throughout the exposure period. The ratio of GSH/GSSG also decreased from 12.8 to 1.2 in ozone exposed trees. Superoxide dismutase levels increased twofold in fumigated plants. After 4 hours of ozone exposure, the photosynthetic rate was approximately half that of controls while glutathione levels and superoxide dismutase activity remained above that of the controls. The elevated antioxidant levels were maintained 21 hours after ozone exposure while photosynthetic rates recovered to about 75% of that of controls. Electron transport and NADPH levels remained unaffected by the treatment. Hence, elevated antioxidant metabolism may protect the photosynthetic apparatus during exposure to ozone.
1 Supported by Environmental Protection Agency grant No. R814197-01-0 to Boyce Thompson Institute, Ithaca, NY, subcontract (BTI 88 No. 1) to R. G. A., Virginia Polytechnic Institute and State University, Blacksburg, VA.
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