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Metabolism and Enzymology

Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants

Protein Purification from Corn Leaves and Immunological Detection

Roussel Uclaf, 102 Route De Noisy, F 93230 Romainville, France, Calgene Inc., Davis, California 95616

We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS polypeptide is 138 kilodaltons. By immunoblot, an SPS antigen was found to accumulate in mature leaves. SPS protein levels remain constant during the day/night cycle. The observed diurnal fluctuation of extractable enzyme activity, therefore, must be caused by modification of the specific activity of SPS in vivo.

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