Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 96:426-431 (1991)
© 1991 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inouhe, M.
Right arrow Articles by Nevins, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inouhe, M.
Right arrow Articles by Nevins, D. J.
Agricola
Right arrow Articles by Inouhe, M.
Right arrow Articles by Nevins, D. J.
Development and Growth Regulation

Inhibition of Auxin-Induced Cell Elongation of Maize Coleoptiles by Antibodies Specific for Cell Wall Glucanases 1

Mashiro Inouhe2 and Donald J. Nevins

Department of Vegetable Crops, University of California, Davis, California 95616

Polyclonal antibodies were raised in rabbits in response to the administration of purified exo- and endoglucanases extracted from cell walls of maize (Zea mays L. B37 x Mo17) coleoptiles. Since the antibodies formed specific conjugates when challenged with the glucanase antigens in immunoblot assays they were employed to evaluate the participation of glucanases in tissue growth. Indole-3-acetic acid induced cell elongation of abraded coleoptile segments was inhibited when the antibodies were supplied as a short term pretreatment (25-200 microgram/milliliter of serum protein). The extent of inhibition of IAA induced cell elongation was additive when endo- and exoglucanase antibodies were applied together. The results suggest that both enzymes have a role in mediating IAA-induced cell elongation. Pretreatment with exo- and endoglucanases antibodies also inhibited IAA induced degradation of noncellulosic beta-D-glucans and the increased level of cellulosic polymers in maize coleoptiles. Antibodies also inhibited the expression of the autohydrolytic degradation of glucans in isolated cell walls. The extent of inhibition was dependent on the antibody concentration applied. The results support the contention that enzymatic processes mediated by exo- and endoglucanases are responsible for cell wall autolytic reactions and that these reactions are linked to the mechanism for expressing auxin induced cell elongation in maize coleoptiles.

2 Permanent address: Dr. Masahiro Inouhe, Department of Biology, Faculty of Science, Ehime University, Matsuyama 790, Japan.

1 Supported, in part, by the National Science Foundation Grant DMB-8505901.




This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
T. Kimpara, T. Aohara, K. Soga, K. Wakabayashi, T. Hoson, Y. Tsumuraya, and T. Kotake
{beta}-1,3 : 1,4-Glucan Synthase Activity in Rice Seedlings under Water
Ann. Bot., August 1, 2008; 102(2): 221 - 226.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Wasaki, T. Shinano, K. Onishi, R. Yonetani, J. Yazaki, F. Fujii, K. Shimbo, M. Ishikawa, Z. Shimatani, Y. Nagata, et al.
Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves
J. Exp. Bot., June 1, 2006; 57(9): 2049 - 2059.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Zhu, S. Chen, S. Alvarez, V. S. Asirvatham, D. P. Schachtman, Y. Wu, and R. E. Sharp
Cell Wall Proteome in the Maize Primary Root Elongation Zone. I. Extraction and Identification of Water-Soluble and Lightly Ionically Bound Proteins
Plant Physiology, January 1, 2006; 140(1): 311 - 325.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Takeda, T. Kotake, N. Nakagawa, N. Sakurai, and D. J. Nevins
Expression and Function of Cell Wall-Bound Cationic Peroxidase in Asparagus Somatic Embryogenesis
Plant Physiology, April 1, 2003; 131(4): 1765 - 1774.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Roulin and U. Feller
Reversible accumulation of (1->3,1->4)-{beta}-glucan endohydrolase in wheat leaves under sugar depletion
J. Exp. Bot., December 1, 2001; 52(365): 2323 - 2332.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D.S. Thompson
Extensiometric determination of the rheological properties of the epidermis of growing tomato fruit
J. Exp. Bot., June 1, 2001; 52(359): 1291 - 1301.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kotake, N. Nakagawa, K. Takeda, and N. Sakurai
Auxin-Induced Elongation Growth and Expressions of Cell Wall-Bound Exo- and Endo-{beta}-Glucanases in Barley Coleoptiles
Plant Cell Physiol., November 1, 2000; 41(11): 1272 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Shpigel, L. Roiz, R. Goren, and O. Shoseyov
Bacterial Cellulose-Binding Domain Modulates in Vitro Elongation of Different Plant Cells
Plant Physiology, August 1, 1998; 117(4): 1185 - 1194.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1991 by the American Society of Plant Biologists