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Plant Physiology 95:990-996 (1991)
© 1991 American Society of Plant Biologists

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Development and Growth Regulation

Carrier-Mediated Uptake of Abscisic Acid by Suspension-Cultured Amaranthus tricolor Cells

Jacqueline Bianco-Colomas, Philippe Barthe, Monique Orlandini and Marie Thérèse Le Page-Degivry

Laboratoire de Physiologie végétale, Université de Nice-Sophia Antipolis, Parc Valrose, 06034 Nice, Cedex, France

Abscisic acid (ABA) uptake by Amaranthus tricolor cell suspensions was found to include both a nonsaturable component and a saturable part with Km of 3.74 ± 0.43 micromolar and an apparent Vmax of 1.5 ± 0.12 nanomoles per gram per minute. These kinetic parameters as well as the uptake by intact cells at 0°C or by frozen and thawed cells, are consistent with operation of a saturable carrier. This carrier-mediated ABA uptake was partially energized by {Delta}pH: it increased as the external pH was lowered to pH 4.0; it decreased after the lowering of the {Delta}pH by the proton ionophore carbonylcyanide-m-chlorophenylhydrazone or after the altering of metabolically maintained pH gradient by metabolic inhibitors (KCN, oligomycin). The carrier is specific for ABA among the plant growth regulators tested, is unaffected by (RS)-trans-ABA and was inhibited by (S)-ABA, (R)-ABA, and also by the ABA analog LAB 173711.




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Copyright © 1991 by the American Society of Plant Biologists