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Metabolism and Enzymology

Translational Modification of an 18 Kilodalton Polypeptide by Spermidine in Rice Cell Suspension Cultures

Plant Molecular Biology, Beltsville Agricultural Research Center (West) Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, Plant Hormone Laboratories, Beltsville Agricultural Research Center (West) Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

When rice (Oryza sativa) cell suspension cultures are grown in the presence of [terminal methylenes-³H]spermidine, label is incorporated in a single polypeptide with a molecular mass of 18 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Preincubation of cell cultures with polyamine biosynthesis inhibitors difluoromethylarginine and difluoromethylornithine, resulted in increased incorporation of the label into the 18 kilodalton polypeptide. In cells in which protein synthesis was arrested by cycloheximide, no label was detected in the 18 kilodalton polypeptide, suggesting a requirement for de novo protein synthesis.

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