Plant Physiol. Drug Metab Dispos
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Plant Physiology 95:197-205 (1991)
© 1991 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Action Spectrum for Resetting the Circadian Phototaxis Rhythm in the CW15 Strain of Chlamydomonas1

I. Cells in Darkness

Takao Kondo, Carl Hirschie Johnson and John Woodland Hastings

National Institute for Basic Biology, Myodaiji, Okazaki, 444, Japan, Department of Biology, Vanderbilt University, Nashville, Tennessee 37235, The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

We have developed protocols for phase shifting the circadian rhythm of Chlamydomonas reinhardtii by light pulses. This paper describes the photobiology of phase-resetting the Chlamydomonas clock by brief (3 seconds to 15 minutes) light pulses administered during a 24 hour dark period. Its action spectrum exhibited two prominent peaks, at 520 and 660 nanometers. The fluence at 520 nanometers required to elicit a 4 hour phase shift was 0.2 millimole photon per square meter, but the pigment that is participating in resetting the clock under these conditions is unknown. The fluence needed at 660 nanomoles to induce a 4 hour phase shift was 0.1 millimole photon per square meter, which is comparable with that needed to induce the typical low fluence rate response of phytochrome in higher plants. However, the phase shift by red light (660 nanometers) was not diminished by subsequent administration of far-red light (730 nanometers), even if the red light pulse was as short as 0.1 second. This constitutes the first report of a regulatory action by red light in Chlamydomonas.

1 Part of this study was carried out under the Cooperative Research Program for the use of the Okazaki Large Spectrograph (project Nos. 86-529, 87-524, and 87-530) of the National Institute for Basic Biology, Okazaki, Japan. This study was also supported in part by grants from the National Institutes of Mental Health to C.H.J. (MH 43836) and J.W.H. (MH 40755).




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