Plant Physiol. Drug Metab Dispos
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Plant Physiology 94:1748-1755 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Separation and Partial Purification of 1,3-beta-Glucan and 1,4-beta-Glucan Synthases from Saprolegnia

Vincent Bulone, Vincent Girard and Michel Fèvre

Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, Université Lyon I, 69622 Villeurbanne Cedex, France, Unité Mixte de Recherches UMR Centre National de la Recherche Scientifique 106 (bât. 405), Université Lyon I, 69622 Villeurbanne Cedex, France

Enriched 1,3-beta-glucan and 1,4-beta-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-beta-Glucan synthases were separated from 1,4-beta-glucan synthases following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-beta-glucan and 1,4-beta-glucan synthases may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, and 50 kD for the 1,3-beta-glucan synthase and 60 kD for the 1,4-beta-glucan synthase).




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[Abstract] [Full Text] [PDF]


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Copyright © 1990 by the American Society of Plant Biologists