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Metabolism and Enzymology

Biosynthetic Origin of Gibberellins A₃ and A₇ in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica¹

School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom, Department of Biology, U.C.L.A., Los Angeles, California 90024

Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A₉ (GA₉) and 2,3-dehydroGA₉ to GA₇; GA₉ was also metabolized to GA₄ in a branch pathway. The preparation from Marah seeds also metabolized GA₅ to GA₃ in high yield; GA₆ was a minor product and was not metabolized to GA₃. Using substrates stereospecifically labeled with deuterium, it was shown that the metabolism of GA₅ to GA₃ and of 2,3-dehydroGA₉ to GA₇ occurs with the loss of the 1-hydrogen. In cultures of Gibberella fujikuroi, mutant B1-41a, [1,2-²H₂]GA₄, was metabolized to [1,2-²H₂]GA₃ with the loss of the 1- and 2-hydrogens. These results provide further evidence that the biosynthetic origin of GA₃ and GA₇ in higher plants is different from that in the fungus Gibberella fujikuroi.

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