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Metabolism and Enzymology

Gibberellin A₃ Is Biosynthesized from Gibberellin A₂₀ via Gibberellin A₅ in Shoots of Zea mays L. ¹

Department of Biology, University of California, Los Angeles, Los Angeles, California 90024, School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom, Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-Ku, Tokyo 113, Japan

[17-¹³C,³H]-Labeled gibberellin A₂₀ (GA₂₀), GA₅, and GA₁ were fed to homozygous normal (+/+), heterozygous dominant dwarf (D8/+), and homozygous dominant dwarf (D8/D8) seedlings of Zea mays L. (maize). ¹³C-Labeled GA₂₉, GA₈, GA₅, GA₁, and 3-epi-GA₁, as well as unmetabolized [¹³C]GA₂₀, were identified by gas chromatography-selected ion monitoring (GC-SIM) from feeds of [17-¹³C, ³H]GA₂₀ to all three genotypes. ¹³C-Labeled GA₈ and 3-epi-G₁, as well as unmetabolized [¹³C]GA₁, were identified by GC-SIM from feeds of [17-¹³C, ³H]GA₁ to all three genotypes. From feeds of [17-¹³C, ³H]GA₅, ¹³C-labeled GA₃ and the GA₃-isolactone, as well as unmetabolized [¹³C]GA₅, were identified by GC-SIM from +/+ and D8/D8, and by full scan GC-MS from D8/+. No evidence was found for the metabolism of [17-¹³C, ³H]GA₅ to [¹³C]GA₁, either by full scan GC-mass spectrometry or by GC-SIM. The results demonstrate the presence in maize seedlings of three separate branches from GA₂₀, as follows: (a) GA₂₀ GA₁ GA₈; (b) GA₂₀ GA₅ GA₃; and (c) GA₂₀ GA₂₉. The in vivo biogenesis of GA₃ from GA₅, as well as the origin of GA₅ from GA₂₀, are conclusively established for the first time in a higher plant (maize shoots).

¹ Financial support is gratefully acknowledged from the National Science Foundation, grant DMB-8506998 (B.O.P.), and the Agricultural and Food Research Council (J.MacM.). These results were originally presented at the IPGSA Symposium held in Calgary, Alberta, Canada, in July 1988.

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