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Plant Physiology 93:1511-1513 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Activity of Ribulose 1,5-Bisphosphate Carboxylase Oxygenase as a Function of Storage Conditions

Bruria Heuer1 and Archie R. Portis, Jr.

Department of Agronomy, University of Illinois, Urbana, Illinois 61801, U.S. Department of Agriculture, Agricultural Research Service, Urbana, Illinois 61801

Optimal storage conditions to retain ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activity were investigated. The soluble spinach (Spinacia oleracea) enzyme was pretreated with its activators, Mg2+ and HCO3–, and then stored for up to 30 days at 4 or –18°C or in liquid N2. Cold inactivation and conformational changes were suggested to be involved during Rubisco storage in the cold, leading to its inactivation. Pretreatment of the enzyme with Mg2+ and CO2 and subsequent storage at either 4°C or in liquid N2 or flushing the samples with N2 and rapid freezing and storage in liquid N2 are recommended as storage procedures. These storage treatments will prevent inactivation, so that full original specific activity will be preserved.

1 Present address: Institute of Soils and Water, ARO, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1990 by the American Society of Plant Biologists