Plant Physiology 93:1504-1510 (1990)
© 1990 American Society of Plant Biologists
Environmental and Stress Physiology
Molecular Cloning and Characterization of Cold-Regulated Genes in Barley 1
Luigi Cattivelli and
Dorothea Bartels
Experimental Institute for Cereal Research, Section of Fiorenzuola d' Arda (PC), 29017, Via S. Protaso, 302, Italy,
Max-Planck-Institut für Züchtungsforschung, Carl von Linne Weg, 10 D-5000 Köln 30, Federal Republic of Germany
Five different cDNA clones have been isolated which are homologous to cold-regulated mRNAs in barley (Hordeum vulgare L.). The analyses of their hybridizations indicate that the transcripts accumulate to different levels during cold-treatment. Hybridization experiments using RNAs isolated from different plant tissues indicate that several cold-regulated genes are expressed in a tissue-specific manner. The expression studies suggest that in barley several different genes are involved in the cold hardening process depending on developmental stages and tissues involved. Homology has been found between the isolated cDNAs and cold-induced transcripts of related cereals. DNA sequence analysis of the clones pT59 and pA086 reveals that the proteins deduced from the longest open reading frame contain arginine rich basic domains.
1 This work was partially supported by the Italian Ministry of Agriculture "Progetto Biotecnologie." L.C. was supported by a fellowship from the Italian Rotary Club and an EMBO short-term fellowship.
This article has been cited by other articles:
|
|
|
|
 
M. Battaglia, Y. Olvera-Carrillo, A. Garciarrubio, F. Campos, and A. A. Covarrubias
The Enigmatic LEA Proteins and Other Hydrophilins
Plant Physiology,
September 1, 2008;
148(1):
6 - 24.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takumi, A. Koike, M. Nakata, S. Kume, R. Ohno, and C. Nakamura
Cold-specific and light-stimulated expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast-targeted protein
J. Exp. Bot.,
October 1, 2003;
54(391):
2265 - 2274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Karlson, K. Nakaminami, T. Toyomasu, and R. Imai
A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins
J. Biol. Chem.,
September 13, 2002;
277(38):
35248 - 35256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ohno, S. Takumi, and C. Nakamura
Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aestivum L.)
J. Exp. Bot.,
December 1, 2001;
52(365):
2367 - 2374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Knight, E. L. Veale, G. J. Warren, and M. R. Knight
The sfr 6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
PLANT CELL,
May 1, 1999;
11(5):
875 - 886.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Crosatti, P. Polverino de Laureto, R. Bassi, and L. Cattivelli
The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein
Plant Physiology,
February 1, 1999;
119(2):
671 - 680.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. L. Steponkus, M. Uemura, R. A. Joseph, S. J. Gilmour, and M. F. Thomashow
Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana
PNAS,
November 24, 1998;
95(24):
14570 - 14575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kaye, L. Neven, A. Hofig, Q.-B. Li, D. Haskell, and C. Guy
Characterization of a Gene for Spinach CAP160 and Expression of Two Spinach Cold-Acclimation Proteins in Tobacco
Plant Physiology,
April 1, 1998;
116(4):
1367 - 1377.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. N. Artus, M. Uemura, P. L. Steponkus, S. J. Gilmour, C. Lin, and M. F. Thomashow
Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance
PNAS,
November 12, 1996;
93(23):
13404 - 13409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
