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Plant Physiology 93:1497-1503 (1990)
© 1990 American Society of Plant Biologists

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Environmental and Stress Physiology

Biosynthesis of Phytosiderophores 1

In Vitro Biosynthesis of 2'-Deoxymugineic Acid from L-Methionine and Nicotianamine

Shinsuke Shojima, Naoko-Kishi Nishizawa, Shinji Fushiya, Shigeo Nozoe, Tomohiro Irifune and Satoshi Mori

Laboratory of Plant Nutrition & Fertilizers, Department of Agricultural Chemistry, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan, Pharmaceutical Institute, Tohoku University, Sendai, Japan

2' -Deoxymugineic acid (DMA), one of mugineic acid-family phytosiderophores (MAs), was synthesized in vitro both from L-methionine and from nicotianamine (NA) with a cell-free system derived from root tips of iron-deficient barley (Hordeum vulgare L.). The reactions producing DMA from NA needed an amino group acceptor (i.e. 2-oxoglutarate, pyruvate, or oxalacetic acid) and a reductant (i.e. NADH or NADPH). The activity of the enzymes to produce NA from L-methionine was the highest at about pH 9. This biosynthetic activity was markedly induced by iron-deficiency stress. The synthesis of NA from S-adenosyl-L-methionine was more efficient than from L-methionine. From the results with the cell-free system reported here, we propose a revised biosynthetic pathway of MAs.

1 Partly supported by Grant-in-Aid (No. 62219004 and No. 62303014) from the Ministry of Education, Science and Culture, Japan




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