This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tsuzuki, M. Articles by Kawaguchi, A. Search for Related Content PubMed PubMed Citation Articles by Tsuzuki, M. Articles by Kawaguchi, A. Agricola Articles by Tsuzuki, M. Articles by Kawaguchi, A.

Environmental and Stress Physiology

Effects of CO₂ Concentration during Growth on Fatty Acid Composition in Microalgae ¹

Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan, Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan

The degree of unsaturation of fatty acids was higher in Chlorella vulgaris 11h cells grown with air (low-CO₂ cells) than in the cells grown with air enriched with 2% CO₂ (high-CO₂ cells). The change in the ratio of linoleic acid to -linolenic acid was particularly significant. This change of the ratio was observed in four major lipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine, and phosphatidylethanolamine). The relative contents of lipid classes were essentially the same both in high-CO₂ and low-CO₂ cells. After high-CO₂ cells were transferred to low CO₂ condition, total amount of fatty acids remained constant but the relative content of -linolenic acid increased during a 6-hour lag phase in growth with concomitant decreases in linoleic and oleic acids. When low-CO₂ cells were transferred to high CO₂ condition, total amount of fatty acids and relative content of oleic acid increased significantly. The amount of -linolenic acid remained almost constant, while the amounts of palmitic, oleic, and linoleic acids increased. Similar, but smaller, changes in fatty acid compositions were observed in two species of green algae Chlamydomonas reinhardtii and Dunaliella tertiolecta. However, no difference was found in Euglena gracilis, Porphyridium cruentum, Anabaena variabilis, and Anacystis nidulans.

This article has been cited by other articles:

				Y. Zheng, W. E. Boeglin, C. Schneider, and A. R. Brash A 49-kDa Mini-lipoxygenase from Anabaena sp. PCC 7120 Retains Catalytically Complete Functionality J. Biol. Chem., February 22, 2008; 283(8): 5138 - 5147. [Abstract] [Full Text] [PDF]