This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Long, D. M. Articles by Oaks, A. Search for Related Content PubMed PubMed Citation Articles by Long, D. M. Articles by Oaks, A. Agricola Articles by Long, D. M. Articles by Oaks, A.

Metabolism and Enzymology

Stabilization of Nitrate Reductase in Maize Roots by Chymostatin ¹

Biology Department, McMaster University, Hamilton, Ontario L8S 4K1 Canada

Nitrate reductase (NR) in maize (Zea mays cv W64A x W182E) roots has been stabilized in vitro by the addition of chymostatin to extraction buffer. Contrary to previous observations, levels of NR were higher in the mature root than in root tip sections when chymostatin was included in the extraction buffer. Two forms of NR were identified, an NADH monospecific NR found mainly in the 1cm root tip and an NAD(P)H bispecific NR found predominantly in mature regions of the root. During the first 10 days of seedling growth, NR activity in the root ranged from 50 to 80% of the activities found in the leaf (a maximum of 2.4 micromoles NO^–₂ produced per hour per gram fresh weight was measured at 4 days).

¹ Research supported by an operating grant from Natural Sciences and Engineering Research Council of Canada (A2818). D. L. was supported by an NSERC postgraduate scholarship.

This article has been cited by other articles:

				A. K. Tobin and T. Yamaya Cellular compartmentation of ammonium assimilation in rice and barley J. Exp. Bot., April 1, 2001; 52(356): 591 - 604. [Abstract] [Full Text] [PDF]