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Plant Physiology 93:1246-1252 (1990)
© 1990 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana1

Ravindra K. Hajela, David P. Horvath, Sarah J. Gilmour and Michael F. Thomashow

Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1325, Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1325, Program in Genetics, Michigan State University, East Lansing, Michigan 48824-1325

We have previously shown that changes in gene expression occur in Arabidopsis thaliana. L. (Heyn) during cold acclimation (SJ Gilmour, RK Hajela, MF Thomashow [1988] Plant Physiol 87: 745-750). Here we report the isolation of cDNA clones of four cold-regulated (cor) genes from Arabidopsis and examine their expression in response to low temperature, abscisic acid (ABA), water stress, and heat shock. The results of Northern analysis indicated that the transcript levels for the four cor genes, represented by clones pHH7.2, pHH28, pHH29, and pHH67, increased markedly between 1 and 4 hours of cold treatment, reached a maximum at about 8 to 12 hours, and remained at elevated levels for as long as the plants were kept in the cold (up to 2 weeks). Returning cold acclimated plants to control temperature resulted in the levels of the cor transcripts falling rapidly to those found in nonacclimated plants; this occurred within 4 hours for the transcripts represented by pHH7.2 and pHH28, and 8 hours for those represented by pHH29 and pHH67. Nuclear run-on transcription assays indicated that the temperature-regulated expression of the cor genes represented by pHH7.2, pHH28, and pHH29 was controlled primarily at the posttranscriptional level while the cor gene represented by pHH67 was regulated largely at the transcriptional level. Northern analysis also indicated that the levels of cor gene transcripts increased in response to both ABA application and water stress, but not to heat shock. The possible significance of cor genes being regulated by both low temperature and water stress is discussed.

1 Supported by grants from the U.S. Department of Agriculture (88-37264-3880), the Michigan Agricultural Experiment Station, and the Michigan Research Excellence Fund.




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