Plant Physiol. Drug Metab Dispos
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Plant Physiology 93:1071-1077 (1990)
© 1990 American Society of Plant Biologists

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Environmental and Stress Physiology

Boron Deficiency in Cultured Pine Cells 1

Quantitative Studies of the Interaction with Ca and Mg

Robert Dixon Teasdale and Dianne Katherine Richards2

Centre for Plant Biotechnology, School of Science and Technology, Bond University, Gold Coast, Qld 4229, Australia

A pronounced interaction between calcium, magnesium, and boron was found in growth studies with Pinus radiata cell cultures. Quantitative isoactivity data for the interaction was analyzed in terms of selected simple and plausible theoretical models. The data was found to be consistent with a model in which a critical acceptor molecule is activated only by binding both Ca and B at separate sites; Mg competitively displaces Ca to inactivate the acceptor. It was found that B is, surprisingly, not bound strongly (Kdiss = 450 ± 80 micromolar) and that the affinity for Ca is two orders of magnitude stronger than for Mg. Therefore only a small proportion of the acceptor will be boronated under natural conditions. Moderate levels of mannitol were found to aggravate B deficiency due to its effective removal by direct chemical complexation. At higher concentrations of mannitol (or other sugars), where osmotic contribution is significant, little B was needed to overcome growth inhibition—a result consistent with B having a primary role in cell wall biosynthesis.

2 Present address: School of Applied Science, Gippsland Institute of Advanced Education, Switchback Road, Churchill, Vic 3842, Australia.

1 This research was supported by the Rural Credits Development Fund grant CAE/8663, Australian Research Grants Scheme award AO8515299, and The Pine Research Fund.






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Copyright © 1990 by the American Society of Plant Biologists