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Metabolism and Enzymology

Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants ¹

Botany Department, Lucknow University, Lucknow-226 007, India

"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(II) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(III) with or without light. The reducing activity was attributed to ascorbic acid and phenols in the plant extracts. The reliability of the Fe(II) iron determination procedures in plants and the interpretation that active Fe is Fe(II) are questionable.

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