This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cogoni, A. Articles by Floris, G. Search for Related Content PubMed PubMed Citation Articles by Cogoni, A. Articles by Floris, G. Agricola Articles by Cogoni, A. Articles by Floris, G.

Metabolism and Enzymology

Hordeum vulgare Seedlings Amine Oxidase

Purification and Properties

Istituto di Chimica Biologica, Università di Cagliari, via della Pineta, 77 09125 Cagliari, Italy

Although no amine oxidase could be detected in crude extracts, the enzyme has been purified to apparent homogeneity from Hordeum vulgare seedlings using ammonium sulfate precipitation and chromatography on DEAE cellulose, Hydroxylapatite, and Sephadex G200 columns. Gel filtration experiments indicate a molecular weight of about 150,000. The pH optimum of the enzyme was found to be 7.5 in potassium phosphate buffer. The spectrum of ultraviolet and visible regions were similar to Cuamine oxidase from Leguminosae.

This article has been cited by other articles:

				D. Walters, T. Cowley, and A. Mitchell Methyl jasmonate alters polyamine metabolism and induces systemic protection against powdery mildew infection in barley seedlings J. Exp. Bot., April 1, 2002; 53(369): 747 - 756. [Abstract] [Full Text] [PDF]