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Plant Physiology 93:657-661 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Purification and Some Properties of a Daucus carota Lectin which Enhances the Activation of Prophenoloxidase by CaCl21

Irene Söderhäll, Annika Bergenstråhle and Kenneth Söderhäll

Department of Physiological Botany, University of Uppsala, Box 540, S-751-21 Uppsala, Sweden

Carrot (Daucus carota) cell cultures were found to secrete a protein, with the molecular weight of 58,000, which could strongly enhance the activation of carrot prophenoloxidase by CaCl2. The protein was isolated from the culture filtrate and purified to electrophoretic homogeneity. The purified protein did also show hemagglutinating activity toward rat as well as rabbit erythrocytes and this activity was inhibitied by N-acetylglucosamine or fetuin.


1 This research has been supported by grants from the Swedish Council for Planning and Coordination of Research.







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Copyright © 1990 by the American Society of Plant Biologists