Plant Physiology 93:623-630 (1990)
© 1990 American Society of Plant Biologists
Metabolism and Enzymology
The S-Methylmethionine Cycle in Lemna paucicostata
S. Harvey Mudd and
Anne H. Datko
Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda, Maryland 20892
The metabolism of S-methylmethionine has been studied in cultures of plants of Lemna paucicostata and of cells of carrot (Daucus carota) and soybean (Glycine max). In each system, radiolabeled S-methylmethionine was rapidly formed from labeled L-methionine, consistent with the action of S-adenosyl-L-methionine:methionine S-methyltransferase, an enzyme which was demonstrated during these studies in Lemna homogenates. In Lemna plants and carrot cells radiolabel disappeared rapidly from S-methylmethionine during chase incubations in nonradioactive media. The results of pulse-chase experiments with Lemna strongly suggest that administered radiolabeled S-methylmethionine is metabolized initially to soluble methionine, then to the variety of compounds formed from soluble methionine. An enzyme catalyzing the transfer of a methyl group from S-methylmethionine to homocysteine to form methionine was demonstrated in homogenates of Lemna. The net result of these reactions, together with the hydrolysis of S-adenosylhomocysteine to homocysteine and adenosine, is to convert S-adenosylmethionine to methionine and adenosine. A physiological advantage is postulated for this sequence in that it provides the plant with a means of sustaining the pool of soluble methionine even when overshoot occurs in the conversion of soluble methionine to S-adenosylmethionine. The facts that the pool of soluble methionine is normally very small relative to the flux into S-adenosylmethionine and that the demand for the latter compound may change very markedly under different growth conditions make it plausible that such overshoot may occur unless the rate of synthesis of S-adenosylmethionine is regulated with exquisite precision. The metabolic cost of this apparent safeguard is the consumption of ATP. This S-methylmethionine cycle may well function in plants other than Lemna, but further substantiating evidence is neeeded.
This article has been cited by other articles:
|
|
|
|
 
G. J. Norton, D. E. Lou-Hing, A. A. Meharg, and A. H. Price
Rice-arsenate interactions in hydroponics: whole genome transcriptional analysis
J. Exp. Bot.,
May 2, 2008;
(2008)
ern097v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bellin, B. Schulz, T. R. Soerensen, F. Salamini, and K. Schneider
Transcript profiles at different growth stages and tap-root zones identify correlated developmental and metabolic pathways of sugar beet
J. Exp. Bot.,
February 1, 2007;
58(3):
699 - 715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Goyer, E. Collakova, Y. Shachar-Hill, and A. D. Hanson
Functional Characterization of a Methionine {gamma}-Lyase in Arabidopsis and its Implication in an Alternative to the Reverse Trans-sulfuration Pathway
Plant Cell Physiol.,
February 1, 2007;
48(2):
232 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Kocsis, P. Ranocha, D. A. Gage, E. S. Simon, D. Rhodes, G. J. Peel, S. Mellema, K. Saito, M. Awazuhara, C. Li, et al.
Insertional Inactivation of the Methionine S-Methyltransferase Gene Eliminates the S-Methylmethionine Cycle and Increases the Methylation Ratio
Plant Physiology,
April 1, 2003;
131(4):
1808 - 1815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Jakubowski and A. Guranowski
Metabolism of Homocysteine-thiolactone in Plants
J. Biol. Chem.,
February 21, 2003;
278(9):
6765 - 6770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tagmount, A. Berken, and N. Terry
An Essential Role of S-Adenosyl-L-Methionine:L-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-L-Selenium- Methionine, the Precursor of Volatile Selenium
Plant Physiology,
October 1, 2002;
130(2):
847 - 856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Roje, S. Y. Chan, F. Kaplan, R. K. Raymond, D. W. Horne, D. R. Appling, and A. D. Hanson
Metabolic Engineering in Yeast Demonstrates That S-Adenosylmethionine Controls Flux through the Methylenetetrahydrofolate Reductase Reaction in Vivo
J. Biol. Chem.,
February 1, 2002;
277(6):
4056 - 4061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hacham, T. Avraham, and R. Amir
The N-Terminal Region of Arabidopsis Cystathionine gamma -Synthase Plays an Important Regulatory Role in Methionine Metabolism
Plant Physiology,
February 1, 2002;
128(2):
454 - 462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Imsande
Selection of Soybean Mutants with Increased Concentrations of Seed Methionine and Cysteine
Crop Sci.,
March 1, 2001;
41(2):
510 - 515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Kocsis and A. D. Hanson
Biochemical Evidence for Two Novel Enzymes in the Biosynthesis of 3-Dimethylsulfoniopropionate in Spartina alterniflora
Plant Physiology,
July 1, 2000;
123(3):
1153 - 1162.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. P. de Souza, C. M. Lytle, M. M. Mulholland, M. L. Otte, and N. Terry
Selenium Assimilation and Volatilization from Dimethylselenoniopropionate by Indian Mustard
Plant Physiology,
April 1, 2000;
122(4):
1281 - 1288.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Rouillon, Y. Surdin-Kerjan, and D. Thomas
Transport of Sulfonium Compounds. CHARACTERIZATION OF THE S-ADENOSYLMETHIONINE AND S-METHYLMETHIONINE PERMEASES FROM THE YEAST SACCHAROMYCES CEREVISIAE
J. Biol. Chem.,
October 1, 1999;
274(40):
28096 - 28105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bourgis, S. Roje, M. L. Nuccio, D. B. Fisher, M. C. Tarczynski, C. Li, C. Herschbach, H. Rennenberg, M. J. Pimenta, T.-L. Shen, et al.
S-Methylmethionine Plays a Major Role in Phloem Sulfur Transport and Is Synthesized by a Novel Type of Methyltransferase
PLANT CELL,
August 1, 1999;
11(8):
1485 - 1498.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Neuhierl, M. Thanbichler, F. Lottspeich, and A. Bock
A Family of S-Methylmethionine-dependent Thiol/Selenol Methyltransferases. ROLE IN SELENIUM TOLERANCE AND EVOLUTIONARY RELATION
J. Biol. Chem.,
February 26, 1999;
274(9):
5407 - 5414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. João Pimenta, T. Kaneta, Y. Larondelle, N. Dohmae, and Y. Kamiya
S-Adenosyl-L-Methionine:L-Methionine S-Methyltransferase from Germinating Barley . Purification and Localization
Plant Physiology,
October 1, 1998;
118(2):
431 - 438.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. G. Kocsis, K. D. Nolte, D. Rhodes, T.-L. Shen, D. A. Gage, and A. D. Hanson
Dimethylsulfoniopropionate Biosynthesis in Spartina alterniflora1 . Evidence That S-Methylmethionine and Dimethylsulfoniopropylamine Are Intermediates
Plant Physiology,
May 1, 1998;
117(1):
273 - 281.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Trossat, B. Rathinasabapathi, E. A. Weretilnyk, T.-L. Shen, Z.-H. Huang, D. A. Gage, and A. D. Hanson
Salinity Promotes Accumulation of 3-Dimethylsulfoniopropionate and Its Precursor S-Methylmethionine in Chloroplasts
Plant Physiology,
January 1, 1998;
116(1):
165 - 171.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. James, K. D. Nolte, and A. D. Hanson
Purification and Properties of S-Adenosyl-L-methionine:L-Methionine S-Methyltransferase from Wollastonia biflora Leaves
J. Biol. Chem.,
September 22, 1995;
270(38):
22344 - 22350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Ranocha, F. Bourgis, M. J. Ziemak, D. Rhodes, D. A. Gage, and A. D. Hanson
Characterization and Functional Expression of cDNAs Encoding Methionine-sensitive and -insensitive Homocysteine S-Methyltransferases from Arabidopsis
J. Biol. Chem.,
May 19, 2000;
275(21):
15962 - 15968.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Thomas, A. Becker, and Y. Surdin-Kerjan
Reverse Methionine Biosynthesis from S-Adenosylmethionine in Eukaryotic Cells
J. Biol. Chem.,
December 22, 2000;
275(52):
40718 - 40724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
