This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ken-Dror, S. Articles by Horwitz, B. A. Search for Related Content PubMed PubMed Citation Articles by Ken-Dror, S. Articles by Horwitz, B. A. Agricola Articles by Ken-Dror, S. Articles by Horwitz, B. A.

Development and Growth Regulation

Altered Phytochrome Regulation of Greening in an aurea Mutant of Tomato ¹

Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel

A brief pulse of red light accelerates chlorophyll accumulation upon subsequent transfer of dark-grown tomato (Lycopersicon esculentum) seedlings to continuous white light. Such potentiation of greening was compared in wild type and an aurea mutant W616. This mutant has been the subject of recent studies of phytochrome phototransduction; its dark-grown seedlings are deficient in phytochrome, and light-grown plants have yellow-green leaves. The rate of greening was slower in the mutant, but the extent (relative to the dark control) of potentiation by the red pulse was similar to that in the wild type. In the wild type, the fluence-response curve for potentiation of greening indicates substantial components in the VLF (very low fluence) and LF (low fluence) ranges. Far-red light could only partially reverse the effect of red. In the aurea mutant, only red light in the LF range was effective, and the effect of red was completely reversed by far-red light. When grown in total darkness, aurea seedlings are also deficient in photoconvertible PChl(ide). Upon transfer to white light, the aurea mutant was defective in both the abundance and light regulation of the light-harvesting chlorophyll a/b binding polypeptide(s) [LHC(II)]. The results are consistent with the VLF response in greening being mediated by phytochrome. Furthermore, the data support the hypothesis that light modulates LHC(II) levels through its control of the synthesis of both chlorophyll and its LHC(II) apoproteins. Some, but not all, aspects of the aurea phenotype can be accounted for by the deficiency in photoreception by phytochrome.

This article has been cited by other articles:

				A. C. McCormac and M. J. Terry Loss of Nuclear Gene Expression during the Phytochrome A-Mediated Far-Red Block of Greening Response Plant Physiology, September 1, 2002; 130(1): 402 - 414. [Abstract] [Full Text] [PDF]

				X. Jin, J. Zhu, and E. Zeiger The hypocotyl chloroplast plays a role in phototropic bending of Arabidopsis seedlings: developmental and genetic evidence J. Exp. Bot., January 1, 2001; 52(354): 91 - 97. [Abstract] [Full Text] [PDF]

				M. J. Terry and R. E. Kendrick Feedback Inhibition of Chlorophyll Synthesis in the Phytochrome Chromophore-Deficient aurea and yellow-green-2 Mutants of Tomato Plant Physiology, January 1, 1999; 119(1): 143 - 152. [Abstract] [Full Text]

				M. J. Terry and R. E. Kendrick The aurea and yellow-green-2 Mutants of Tomato Are Deficient in Phytochrome Chromophore Synthesis J. Biol. Chem., August 30, 1996; 271(35): 21681 - 21686. [Abstract] [Full Text] [PDF]