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Metabolism and Enzymology

Estimation of Carbon and Nitrogen Allocation during Stalk Elongation by ¹³C and ¹⁵N Tracing in Zea mays L. ¹

Laboratoire du Métabolisme et de la Nutrition des Plantes, INRA, 78026 Versailles Cedex, France, Laboratoire de Structure et Métabolisme des Plantes, C.N.R.S. (U.R.A. 1128), Université de Paris-Sud, Bat. 430, 91405 Orsay cedex, France, Laboratoire de Biogéochimie Isotopique, INRA-Université P. et M. Curie, C.N.R.S. (U.R.A. 196), T26, 4E, 4 place Jussieu, 75252 Paris cedex 05, France

Zea mays L. (cv Dea) plants grown to the stage of stalk elongation, were allowed to assimilate ¹³CO₂ and ¹⁵N-nitrates from 45 to 53 days after sowing. Isotopic abundances in labeled nutrients were slightly enriched compared to natural abundances. The new C in plant was acropetally distributed and the new N was preferentially accumulated in the sheath and stalk in the medium region. C input was 25-fold higher than N input. The new C in total plant C was 20%, whereas it was 10% for N. The stalk acted as a major sink because it accumulated, respectively, 27.5 and 47.5% of the C and N inputs. The new C in soluble carbohydrates was 76% in growing organs (upper stalk) and only 39% in source leaves, whereas it was 43% and 13% in starch, respectively. New N in nitrates+amino-acids spanned in the range from 20% (leaf) to 50% (stalk). New C and N in soluble proteins were, respectively, 13.4 and 3.8% in leaves, 8.8 and 9.6% in stalk, and 8.7 and 14.3% in roots. In the middle stalk and leaves, the proteins and carbohydrates represent an equivalent C and N source for remobilization.

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