Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 92:246-253 (1990)
© 1990 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reckmann, U.
Right arrow Articles by Raschke, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reckmann, U.
Right arrow Articles by Raschke, K.
Agricola
Right arrow Articles by Reckmann, U.
Right arrow Articles by Raschke, K.
Metabolism and Enzymology

Rubisco Activity in Guard Cells Compared with the Solute Requirement for Stomatal Opening 1

Udo Reckmann, Renate Scheibe and Klaus Raschke

Pflanzenphysiologisches Institut und Botanischer Garten der Universität Göttingen, Untere Karspüle 2, 3400 Göttingen, West Germany, Botanisches Institut der Universität Bayreuth, Lehrstuhl Pflanzenphysiologie, Universitätsstrasse 30, 8580 Bayreuth, West Germany

We investigated whether the reductive pentose phosphate path in guard cells of Pisum sativum had the capacity to contribute significantly to the production of osmotica during stomatal opening in the light. Amounts of ribulose 1,5-bisphophate carboxylase/oxygenase (Rubisco) were determined by the [14C]carboxyarabinitol bisphosphate assay. A guard cell contained about 1.2 and a mesophyll cell about 324 picograms of the enzyme; the ratio was 1:270. The specific activities of Rubisco in guard cells and in mesophyll cells were equal; there was no indication of a specific inhibitor of Rubisco in guard cells. Rubisco activity was 115 femtomol per guard-cell protoplast and hour. This value was different from zero with a probability of 0.99. After exposure of guard-cell protoplasts to 14CO2 for 2 seconds in the light, about one-half of the radioactivity was in phosphorylated compounds and <10% in malate. Guard cells in epidermal strips produced a different labelling pattern; in the light, <10% of the label was in phosphorylated compounds and about 60% in malate. The rate of solute accumulation in intact guard cells was estimated to have been 900 femto-osmol per cell and hour. If Rubisco operated at full capacity in guard cells, and hexoses were produced as osmotica, solutes could be supplied at a rate of 19 femto-osmol per cell and hour, which would constitute 2% of the estimated requirement. The capacity of guard-cell Rubisco to meet the solute requirement for stomatal opening in leaves of Pisum sativum is insignificant.

1 Supported by grants from the Deutsche Forschungsgemeinschaft to K. R. (Ra 122/11) and R. S. (Sche 217/3).




This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Tallman
Are diurnal patterns of stomatal movement the result of alternating metabolism of endogenous guard cell ABA and accumulation of ABA delivered to the apoplast around guard cells by transpiration?
J. Exp. Bot., September 1, 2004; 55(405): 1963 - 1976.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Stadler, M. Buttner, P. Ache, R. Hedrich, N. Ivashikina, M. Melzer, S. M. Shearson, S. M. Smith, and N. Sauer
Diurnal and Light-Regulated Expression of AtSTP1 in Guard Cells of Arabidopsis
Plant Physiology, October 1, 2003; 133(2): 528 - 537.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Lawson, K. Oxborough, J. I. L. Morison, and N. R. Baker
The responses of guard and mesophyll cell photosynthesis to CO2, O2, light, and water stress in a range of species are similar
J. Exp. Bot., July 1, 2003; 54(388): 1743 - 1752.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Lawson, K. Oxborough, J. I.L. Morison, and N. R. Baker
Responses of Photosynthetic Electron Transport in Stomatal Guard Cells and Mesophyll Cells in Intact Leaves to Light, CO2, and Humidity
Plant Physiology, January 1, 2002; 128(1): 52 - 62.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Tominaga, T. Kinoshita, and K.-i. Shimazaki
Guard-Cell Chloroplasts Provide ATP Required for H+ Pumping in the Plasma Membrane and Stomatal Opening
Plant Cell Physiol., August 1, 2001; 42(8): 795 - 802.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Zhang, L. Zhang, F. Dong, J. Gao, D. W. Galbraith, and C.-P. Song
Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
Plant Physiology, August 1, 2001; 126(4): 1438 - 1448.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. H. Outlaw Jr. and X. De Vlieghere-He
Transpiration Rate. An Important Factor Controlling the Sucrose Content of the Guard Cell Apoplast of Broad Bean
Plant Physiology, August 1, 2001; 126(4): 1716 - 1724.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Ritte, J. Rosenfeld, K. Rohrig, and K. Raschke
Rates of Sugar Uptake by Guard Cell Protoplasts of Pisum sativum L. Related to the Solute Requirement for Stomatal Opening
Plant Physiology, October 1, 1999; 121(2): 647 - 656.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1990 by the American Society of Plant Biologists