Plant Physiol.
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Plant Physiology 90:799-805 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Subcellular Distribution of Enzymes of Glycolate Metabolism in the Alga Cyanidium caldarium1

Wolfgang Gross2 and Harry Beevers

Biology Department, University of California Santa Cruz, Santa Cruz, California 95064

The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and glutamate-glyoxylate aminotransferase along with catalase were present in the peroxisomal fraction (density: 1.23 grams per cubic centimeter). Serine hydroxymethyltransferase was found in the mitochondrial fraction (density: 1.18 grams per cubic centimeter). In contrast to the observations in green leaves of higher plants, the enzymes for the conversion of serine to glycerate (serine-glyoxylate aminotransferase and hydroxypyruvate reductase) were found only in the soluble fraction of the gradient. The partial characterization of enzymes from Cyanidium participating in glycolate metabolism revealed only slight differences from the corresponding enzymes from higher plants. The phylogenetic implications of the observed similarities between the enigmatic alga Cyanidium and higher plants are discussed.

2 Present address: Department of Botany, North Carolina State University, Raleigh, NC 27695.

1 This work was supported by National Science Foundation (grant PCM 84-03542) and a postdoctoral fellowship (Feodor-Lynen-Program) from the Alexander-von-Humboldt foundation (Federal Republic of Germany) to W. G.






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Copyright © 1989 by the American Society of Plant Biologists