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Plant Physiology 82:485-487 (1986)
© 1986 American Society of Plant Biologists

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Kinetic Studies on the Control of the Bean Rust Fungus (Uromyces phaseoli L.) by an Inhibitor of Polyamine Biosynthesis 1

M. Venkat Rajam, Leonard H. Weinstein and Arthur W. Galston

Botany Department, Kakatiya University, Warangal 506 009 AP, India, Boyce Thompson Institute, Ithaca, New York 14853, Department of Biology, Yale University, P. O. Box 6666, New Haven, Connecticut 06511

{alpha}-Difluoromethylornithine (DFMO), a specific and irreversible inhibitor of the polyamine biosynthetic enzyme ornithine decarboxylase, effectively inhibits mycelial growth of several phytopathogenic fungi on defined media in vitro and provides systemic protection of bean plants against infection by Uromyces phaseoli L. race 0 (MV Rajam, AW Galston 1985 Plant Cell Physiol 26: 683-692; MV Rajam et al. 1985 Proc Natl Acad Sci USA 82: 6874-6878). We now find that application of 0.5 millimolar DFMO to unifoliolate leaves of Pinto beans up to 3 days after inoculation with uredospores of U. phaseoli completely inhibits the growth of the pathogen, while application 4 or 5 days after inoculation results in partial protection against the pathogen. Spores do not germinate on the surface of unifoliolate leaves treated with DFMO 1 day before infection, but addition of spermidine to the DFMO treatments partially reverses the inhibitory effect. The titer of polyamines in bean plants did not decline after DFMO treatment; rather, putrescine and spermidine contents actually rose, probably due to the known but paradoxical stimulation of arginine decarboxylase activity by DFMO.

1 Supported by grants from the NIH and BARD (US-Israel Binational Research and Development Fund) to A.W.G. M.V.R. is grateful to the Ministry of Education and Culture, Government of India, New Delhi for the award of a National Scholarship for study abroad 1982-1983.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists