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Plant Physiology 82:379-383 (1986)
© 1986 American Society of Plant Biologists

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Articles

Biosynthesis of the Fucose-Containing Xyloglucan Nonasaccharide by Pea Microsomal Membranes 1

Anne Camirand and Gordon Maclachlan

Biology Department, McGill University, 1205 Avenue du Docteur Penfield, Montreal, QC, Canada, H3A 1B1

Pea microsomal membranes catalyze the transfer of [14C]fucose (Fuc) from GDP-[U-14C]fucose, with or without added unlabeled UDP-glucose (Glc), UDP-xylose (Xyl) or UDP-galactose (Gal), to an insoluble product with properties characteristic of xyloglucan. After digestion of the ethanol-insoluble pellet with Streptomyces griseus endocellulase, [14C] fucose residues occur exclusively in a fragment corresponding in size to the xyloglucan nonasaccharide, Glc4 Xyl3 Gal Fuc. This fragment contains a single labeled fucose residue per oligomer, {alpha}-linked in a terminal nonreducing position. By comparison, in incubations where GDP-[14C] fucose is absent and replaced by UDP-[3H]xylose, the maximum size of labeled oligosaccharide found following cellulase digestion of products is an octasaccharide. In the presence of both GDP-[14C]fucose and UDP-[3H]xylose, a nonasaccharide containing the two labels is produced. Fucose and xylose residues are transferred within a few minutes to acceptor molecules of molecular weight up to 300,000. Such products do not elongate detectably over 60 minutes of incubation. The data support the conclusion that the nonasaccharide subunit of xyloglucan may be generated in vitro by transfucosylation to preformed acceptor chains, and that its synthesis is dependent on the inclusion of exogenous GDP-fucose.

1 Supported by grants to G. M. and graduate scholarships to A. C. from the Natural Sciences and Engineering Research Council of Canada and le Programme de Formation des Chercheurs et d'Action Concertée du Québec.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists