This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fawley, M. W. Articles by Grossman, A. R. Search for Related Content PubMed PubMed Citation Articles by Fawley, M. W. Articles by Grossman, A. R. Agricola Articles by Fawley, M. W. Articles by Grossman, A. R.

Articles

Polypeptides of a Light-Harvesting Complex of the Diatom Phaeodactylum tricornutum Are Synthesized in the Cytoplasm of the Cell as Precursors ¹

Department of Botany, Miami University, Oxford, Ohio 45056, Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305

A light-harvesting fucoxanthin-chlorophyll a/c-protein complex has been isolated from the diatom Phaeodactylum tricornutum by detergent extraction of thylakoid membranes coupled with sucrose density gradient centrifugation. The isolated complex was devoid of photochemical activity and displayed spectral characteristics consistent with light harvesting function. It has three major polypeptides of apparent molecular weights 18,000, 19,000, and 19,500 as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Using protein synthesis inhibitors, these polypeptides were shown to be synthesized on 80S cytoplasmic ribosomes. Antibodies raised to a mixture of the 19,000 and 19,500 dalton components of the complex were used to demonstrate structural similarity among the three polypeptide components. Immunoprecipitation from primary translation products synthesized in a reticulocyte lysate system primed with P. tricornutum poly(A) RNA, indicates that the polypeptide components are synthesized as precursors 3,000 to 5,000 daltons larger than the mature polypeptides.

This article has been cited by other articles:

				Y. Fujita and K. Ohki On the 710 nm Fluorescence Emitted by the Diatom Phaeodactylum tricornutum at Room Temperature Plant Cell Physiol., April 15, 2004; 45(4): 392 - 397. [Abstract] [Full Text] [PDF]

				J. J. Hanten and S. K. Pierce Synthesis of Several Light-Harvesting Complex I Polypeptides Is Blocked by Cycloheximide in Symbiotic Chloroplasts in the Sea Slug, Elysia chlorotica (Gould): A Case for Horizontal Gene Transfer Between Alga and Animal? Biol. Bull., August 1, 2001; 201(1): 34 - 44. [Abstract] [Full Text] [PDF]