Plant Physiol. Drug Metab Dispos
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Plant Physiology 80:982-987 (1986)
© 1986 American Society of Plant Biologists

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Articles

Identification of a Highly Conserved Domain on Phytochrome from Angiosperms to Algae 1

Marie-Michèle Cordonnier2, Hubert Greppin and Lee H. Pratt

Laboratoire de Physiologie Végétale, Pavillon des Isotopes, 20 Boulevard d'Yvoy, CH-1211 Genève 4, Botany Department, University of Georgia, Athens, Georgia 30602

A monoclonal antibody (Pea-25) directed to phytochrome from etiolated peas (Pisum sativum L., cv Alaska) binds to an antigenic domain that has been highly conserved throughout evolution. Antigenic cross-reactivity was evaluated by immunoblotting sodium dodecyl sulfate sample buffer extracts prepared from lyophilized tissue samples or freshly harvested algae. Pea-25 immunostained an approximately 120-kilodalton polypeptide from a variety of etiolated and green plant tissues, including both monocotyledons and dicotyledons. Moreover, Pea-25 immunostained a similarly sized polypeptide from the moss Physcomitrella, and from the algae Mougeotia, Mesotaenium, and Chlamydomonas. Because Pea-25 is directed to phytochrome, and because it stains a polypeptide about the size of oat phytochrome, it is likely that Pea-25 is detecting phytochrome in each case. The conserved domain that is recognized by Pea-25 is on the nonchromophore bearing, carboxyl half of phytochrome from etiolated oats. Identification of this highly conserved antigenic domain creates the potential to expand investigations of phytochrome at a cellular and molecular level to organisms, such as Chlamydomonas, that offer unique experimental advantages.

2 Present address: Biotechnology Research, CIBA-GEIGY Corporation, Research Triangle Park, NC 27709-2257.

1 Supported by Swiss National Funds grant 3:292-0:82 and National Science Foundation grants PCM-8315840 and PCM-8315882.




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S. S. H. Husaineid, R. A. Kok, M. E. L. Schreuder, M. Hanumappa, M.-M. Cordonnier-Pratt, L. H. Pratt, L. H. W. van der Plas, and A. R. van der Krol
Overexpression of homologous phytochrome genes in tomato: exploring the limits in photoperception
J. Exp. Bot., February 1, 2007; 58(3): 615 - 626.
[Abstract] [Full Text] [PDF]


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Copyright © 1986 by the American Society of Plant Biologists