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The Effects of Streptomycin, Desiccation, and UV Radiation on Ice Nucleation by Pseudomonas viridiflava

Appalachian Fruit Research Station, Kearneysville, West Virginia 25430

Streptomycin (100 micrograms per milliliter), desiccation (over CaSO₄), and ultraviolet radiation (4500 microwatts per square centimeter at 254 nanometers for 15 minutes) reduced ice nucleation activity by Pseudomonas viridiflava strain W-1 as determined by freezing drops of the bacterial suspensions. Highest residual ice nucleation activity by dead cells was obtained by desiccation, although no freezing above –3.5°C was detected. The rate and extent of loss of ice nucleation activity following streptomycin and ultraviolet treatment was affected by preconditioning temperature. At 21°C and above, loss of activity by dead cells was rapid and irreversible.