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Plant Physiology 80:859-862 (1986)
© 1986 American Society of Plant Biologists

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Detection of the Messenger RNA Encoding for the Ferredoxin-Dependent Glutamate Synthase in Maize Leaf

Bernard Commere, Jean Vidal, Akira Suzuki, Pierre Gadal and Michel Caboche

Laboratoire de Biologie Cellulaire, INRA CNRA, Route de St Cyr, 78000 Versailles, France, Laboratoire de Physiologie Végétale Moléculaire, Bât 430, Université de Paris-Sud, 91405 Orsay Cedex, France, Department of Biology, Mac Master University, 1280 Main Street West, Hamilton Ontario L8J 4K1, Canada

Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), glutamate oxoglutarate aminotransferase (glutamate synthase) (GOGAT) messenger RNA was extracted from maize (Zea mays L.) leaves and partially purified through oligo(dT)-cellulose chromatography and ultracentrifugation in a sucrose gradient. mRNA were translated in vitro using a reticulocyte system. The glutamate synthase subunit was characterized by immunoprecipitation with antibodies raised against the rice (Oryza sativa L.) ferredoxin-glutamate synthase. The in vitro synthesized protein and the 145 kilodaltons genuine maize leaf subunit of GOGAT were found to comigrate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis experiments.






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Copyright © 1986 by the American Society of Plant Biologists