Plant Physiol. Drug Metab Dispos
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Plant Physiology 80:829-833 (1986)
© 1986 American Society of Plant Biologists

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Articles

The Immunologically Conserved Phycobilisome-Thylakoid Linker Polypeptide 1

Barbara A. Zilinskas and Dawn A. Howell

Department of Biochemistry and Microbiology, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey 08903

We have isolated phycobilisomes from two classes of red algae, several subdivisions of the cyanobacteria, and the cyanelles of Cyanophora paradoxa. In addition to the major light harvesting biliproteins, these phycobilisomes also contain several other polypeptides, the largest of which ranges from 75 to 120 kilodaltons in the different species surveyed. This protein, previously isolated and characterized from three species, was shown to be the final emitter of excitation energy in phycobilisomes and is also thought to be involved in the attachment of the phycobilisomes to the thylakoid membrane. We have obtained polyclonal antibodies to the 95 kilodalton polypeptide isolated from phycobilisomes of the cyanobacterium, Nostoc sp. This protein shares no common antigenic determinants with either the {alpha} or beta subunits of allophycocyanin, or any of the other biliproteins, as determined by the sensitive Western immunoblotting technique. However, this antiserum cross-reacts with the highest molecular weight polypeptide of all the rhodophytan and cyanobacterial phycobilisomes tested. That these proteins are immunologically related, but are unrelated to other biliproteins, is reminiscent of previous immunological studies of biliproteins which showed that while the three major spectroscopically distinct classes of biliproteins (phycoerythrin, phycocyanin, and allophycocyanin) shared no common antigenic determinants, there was a strong antigenic determinant to specific biliprotein classes which crossed taxonomic divisions.

1 Supported in part by the Science and Education Administration of the United States Department of Agriculture under Grant No. 85-CRCR-1-1562 from the Competitive Research Grants Office. New Jersey Agricultural Experiment Station, Publication No. D-01104-2-85, supported by State Funds and by the United States Hatch Act.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists