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Plant Physiology 80:1012-1019 (1986)
© 1986 American Society of Plant Biologists

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Articles

Structure of Plant Cell Walls 1

XVIII. An Analysis of the Extracellular Polysaccharides of Suspension-Cultured Sycamore Cells

Thomas T. Stevenson2, Michael McNeil3, Alan G. Darvill2 and Peter Albersheim2

Department of Chemistry, Campus Box 215, University of Colorado, Boulder, Colorado 80309

The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified {alpha}-1,4-endopolygalacturonase (EPG). The EPG-treated SEPS were fractionated by anion-exchange and gelpermeation chromatography. The following polysaccharides were found: xyloglucan, arabinoxylan, at least two arabinogalactans, a rhamnogalacturonan-II-like polysaccharide, and a polygalacturonic acid-rich polysaccharide. The oligogalacturonide fragments expected from EPG-digested homogalacturonan were also identified. Evidence was obtained for the presence of a rhamnogalacturonan-I-like polysaccharide. All of the above polysaccharides have been isolated from or are believed to be present in sycamore cell walls. Furthermore, all of the noncellulosic polysaccharides known to be present in sycamore cell-walls appear to be present in the SEPS.

2 Present address: Complex Carbohydrate Research Center, Richard B. Russell Agricultural Research Center, P. O. Box 5677, Athens, GA 30613.

3 Present address: Department of Microbiology, Colorado State University, Fort Collins, CO 80523.

1 Supported by United States Department of Energy Contract No. DE-AC02-76ER01426. This paper is number XVIII in a series, "Structure of Plant Cell Walls." For the preceding paper see Ref. 24.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists