This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Esen, A. Search for Related Content PubMed PubMed Citation Articles by Esen, A. Agricola Articles by Esen, A.

Articles

Separation of Alcohol-Soluble Proteins (Zeins) from Maize into Three Fractions by Differential Solubility

Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

The prolamin of maize (Zea mays L.), zein, was extracted from endosperm meal with 60% (v/v) 2-propanol/1% (v/v) 2-mercaptoethanol either directly or subsequent to extraction with 90% (v/v) 2-propanol. The zein extracted with 90% 2-propanol was essentially made up of 20 to 24 kilodalton polypeptides (-zein) while that extractable with 60% 2-propanol/1% 2-mercaptoethanol contained, in addition to -zein, 17 to 18 kilodalton methionine-rich polypeptides and a 27 kilodalton proline-rich polypeptide. While zein was separated into three fractions by differential solubility in 90% 2-propanol and 30% 2-propanol/30 millimolar sodium acetate (pH 6) using two different fractionation protocols. Each of the three solubility fractions (SF1, SF2, and SF3) had a unique polypeptide composition. Based on results obtained from two inbreds, K55 and W64A, the SF1 constituted 75 to 80% of the total zein and included as major components 20 to 24 kilodalton polypeptides and a minor 10 kilodalton polypeptide. The SF2 made up 10 to 15% of the total zein and included exclusively 17 to 18 kD methionine-rich polypeptides. A 27 kilodalton proline-rich component constituted the SF3 and contributed 5 to 10% to total zein.

This article has been cited by other articles:

				G. Lund, M. Lauria, P. Guldberg, and S. Zaina Duplication-Dependent CG Suppression of the Seed Storage Protein Genes of Maize Genetics, October 1, 2003; 165(2): 835 - 848. [Abstract] [Full Text] [PDF]

				J. A. Yunes, A. L. Vettore, M. J. da Silva, A. Leite, and P. Arruda Cooperative DNA Binding and Sequence Discrimination by the Opaque2 bZIP Factor PLANT CELL, November 1, 1998; 10(11): 1941 - 1956. [Abstract] [Full Text] [PDF]

				J. Wallace, G Galili, E. Kawata, R. Cuellar, M. Shotwell, and B. Larkins Aggregation of lysine-containing zeins into protein bodies in Xenopus oocytes Science, April 29, 1988; 240(4852): 662 - 664. [Abstract] [PDF]