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Oxygen Exchange in Ulva Using a Bare Platinum Electrode with 4 Microsecond Saturating Light Flashes

Department of Physics, Simon Fraser University, Burnaby, B.C., Canada V5A 1S6, Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada V5A 1S6

Illuminating algae (Ulva species) on a bare platinum electrode with 4 microseconds of saturating light flashes leads to the familiar four-step oscillatory pattern of O₂ exchange. However, it appears that this O₂ exchange is a composite of O₂ evolution and endogenous O₂ uptake in the algae. We have added the O₂ uptake component to the O₂ exchange curve in order to obtain the corrected O₂ evolution flash sequence which shows a similar four-step oscillation as reported previously, but with negligible damping over the first three oscillations.