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Plant Physiology 80:310-314 (1986)
© 1986 American Society of Plant Biologists

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Articles

Development of (1->3,1->4)-beta-D-Glucan Endohydrolase Isoenzymes in Isolated Scutella and Aleurone Layers of Barley (Hordeum vulgare) 1

I. Montgomery Stuart2, Lin Loi and Geoffrey B. Fincher

Department of Biochemistry, La Trobe University, Bundoora, Victoria, 3083, Australia

An immunological assay has been used to investigate the synthesis of (1->3,1->4)-beta-glucanase (EC 3.2.1.73) isoenzymes from isolated barley aleurone layers and scutella. Enzyme release from both tissues is enhanced by 1 micromolar gibberellic acid and 10 millimolar Ca2+, although increases induced by gibberellic acid are observed only in the presence of Ca2+. Isoenzyme I is synthesized predominantly in the scutellum, while isoenzyme II is synthesized exclusively in the aleurone. A third, putative isoenzyme III has been detected in significant proportions in scutellar secretions and may also be secreted from aleurone layers. Both gibberellic acid and Ca2+ appear to preferentially enhance isoenzyme II secretion from the aleurone and isoenzyme III secretion from scutella. The patterns of isoenzyme secretion are suggestive of tissue-specific differences in expression of the genes which code for (1->3,1->4)-beta-glucanase isoenzymes. Qualitatively similar results were obtained with barley cultivars harvested in Australia and North America.

2 Recipient of a LaTrobe University Research Scholarship.

1 Supported by grants from the Australian Research Grants Scheme and the Barley Industry Research Council (to G.B.F.).




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S. Ritchie, A. McCubbin, G. Ambrose, T.-h. Kao, and S. Gilroy
The Sensitivity of Barley Aleurone Tissue to Gibberellin Is Heterogeneous and May Be Spatially Determined
Plant Physiology, June 1, 1999; 120(2): 361 - 370.
[Abstract] [Full Text]


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Copyright © 1986 by the American Society of Plant Biologists