Plant Physiology 80:99-104 (1986)
© 1986 American Society of Plant Biologists
Articles
Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L. 1
Krystyna Bialek and
Jerry D. Cohen
United States Department of Agriculture, Plant Hormone Laboratory, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705,
Department of Botany, University of Maryland, College Park, Maryland 20742
A major indole-3-acetic acid conjugate from Phaseolus vulgaris seed has been isolated and partially characterized. It is a 3 kilodalton peptide with apparently 2 indole-3-acetyl moieties in amide linkage per peptide. The indole-3-acetic acid component was identified by gas chromatography-mass spectrometry and the peptide characterized by polyacrylamide gel electrophoresis, by amino acid analysis using dabsyl derivatives and by its Fourier transform-infrared spectrum. This is the first higher molecular weight amide-linked indole-3-acetic acid conjugate to be characterized from higher plants.
1 Carried out under the cooperative agreement No. 58-3244-3-452 of the Agricultural Research Service, United States Department of Agriculture and the University of Maryland.
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