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Plant Physiology 80:91-98 (1986)
© 1986 American Society of Plant Biologists

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Articles

Structural Requirements for the Binding of Phenylglycosides to the Surface of Protoplasts 1

Eugene A. Nothnagel and J. L. Lyon

Department of Botany and Plant Sciences, University of California, Riverside, California 92521

A variety of phenylglycosides have been synthesized and tested for binding to the surface of protoplasts from suspension-cultured cells of "Paul's Scarlet" rose (Rosa sp.). Multivalent phenylglycosides in the form of Yariv antigens (1,3,5,-tri-[p-glycosyloxyphenylazo]-2,4,6,-trihydroxybenzene) agglutinated the protoplasts. Fluorescence-labeled derivatives of other monovalent and polyvalent phenyl-beta-glycosides did not bind to the protoplast surface. Agglutination was induced by Yariv antigens only if these probes contained beta-anomeric, O-glycosidic linkages. Yariv antigens containing {alpha}-anomeric or thio-glycosidic linkages did not agglutinate protoplasts. These same structural features of Yariv antigens were also required for the precipitation of gum arabic-Yariv antigen complexes. The results suggest that plasma membranes of "Paul's Scarlet" rose protoplasts contain arabinogalactan-proteins that interact with phenyl-beta-glycosides. The results further show that binding at these plasma membrane sites is not solely dependent upon the carbohydrate portion of single phenylglycosides, but may also require specific spatial orientations of adjacent phenylglycosides.

1 Supported by the United States Department of Agriculture Competitive Research Grants Program under Agreement No. 85-CRCR-1-1607, by an Intramural Research Grant (5-547570-07427) from the University of California, Riverside, and by BRSG SO7 RR07010-19 awarded by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists