This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vick, B. A. Articles by Zimmerman, D. C. Search for Related Content PubMed PubMed Citation Articles by Vick, B. A. Articles by Zimmerman, D. C. Agricola Articles by Vick, B. A. Articles by Zimmerman, D. C.

Articles

Characterization of 12-Oxo-Phytodienoic Acid Reductase in Corn

The Jasmonic Acid Pathway

United States Department of Agriculture, Agricultural Research Service, State University Station, Fargo, North Dakota 58105, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota 58105

12-Oxo-phytodienoic acid reductase, an enzyme of the biosynthetic pathway that converts linolenic acid to jasmonic acid, has been characterized from the kernel and seedlings of corn (Zea mays L.). The molecular weight of the enzyme, estimated by gel filtration, was 54,000. Optimum enzyme activity was observed over a broad pH range, from pH 6.8 to 9.0. The enzyme had a K_m of 190 micromolar for its substrate, 12-oxo-phytodienoic acid. The preferred reductant was NADPH, for which the enzyme exhibited a K_m of 13 micromolar, compared with 4.2 millimolar for NADH. Reductase activity was low in the corn kernel but increased five-fold by the fifth day after germination and then gradually declined.

This article has been cited by other articles:

				Y. Ishiga, A. Funato, T. Tachiki, K. Toyoda, T. Shiraishi, T. Yamada, and Y. Ichinose Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen Plant Cell Physiol., October 15, 2002; 43(10): 1210 - 1220. [Abstract] [Full Text] [PDF]

				F. Schaller, P. Hennig, and E. W. Weiler 12-Oxophytodienoate-10,11-Reductase: Occurrence of Two Isoenzymes of Different Specificity against Stereoisomers of 12-Oxophytodienoic Acid Plant Physiology, December 1, 1998; 118(4): 1345 - 1351. [Abstract] [Full Text]

				H. Weber, B. A. Vick, and E. E. Farmer Dinor-oxo-phytodienoic acid: A new hexadecanoid signal in the jasmonate family PNAS, September 16, 1997; 94(19): 10473 - 10478. [Abstract] [Full Text] [PDF]

				B. A. Stelmach, A. Muller, P. Hennig, S. Gebhardt, M. Schubert-Zsilavecz, and E. W. Weiler A Novel Class of Oxylipins, sn1-O-(12-Oxophytodienoyl)-sn2-O-(hexadecatrienoyl)-monogalactosyl Diglyceride, from Arabidopsis thaliana J. Biol. Chem., April 13, 2001; 276(16): 12832 - 12838. [Abstract] [Full Text] [PDF]

				A. Stintzi and J. Browse The Arabidopsis male-sterile mutant, opr3, lacks the 12-oxophytodienoic acid reductase required for jasmonate synthesis PNAS, September 12, 2000; 97(19): 10625 - 10630. [Abstract] [Full Text] [PDF]