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Plant Physiology 76:293-296 (1984)
© 1984 American Society of Plant Biologists

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Articles

Deferral of Senescence and Abscission by Chemical Inhibition of Ethylene Synthesis and Action in Bean Explants 1

Mosbah M. Kushad2 and B. W. Poovaiah

Department of Horticulture and Landscape Architecture, Washington State University, Pullman, Washington 99164-6414

Three compounds known to inhibit ethylene synthesis and/or action were compared for their ability to delay senescence and abscission of bean explants (Phaseolus vulgaris L. cv Contender). Aminoethoxyvinyl-glycine (AVG), AgNO3, and sodium benzoate were infiltrated into the petiole explants. Their effect on abscission was monitored by measuring the force required to break the abscission zone, and their effect on senescence was followed by measuring chlorophyll and soluble protein in the distal (pulvinus) sections. AVG at concentrations between 1 and 100 micromolar inhibited ethylene synthesis by about 80 to 90% compared to the control during sampling periods of 24 and 48 hours after treatment. This compound also delayed the development of abscission and senescence. Treatment with AgNO3 at concentrations between 1 and 100 micromolar progressively reduced ethylene production, but to a lesser extent than AVG. The effects of AgNO3 on senescence and abscission were quite similar to those of AVG. Sodium benzoate at 50 micromolar to 5 millimolar did not inhibit ethylene synthesis during the first 24 hours, but appreciably inhibited ethylene synthesis 48 hours after treatment. It also delayed the development of abscission and senescence. The effects of AVG, Ag+, and sodium benzoate suggest that ethylene could play a major role in both the senescence induction phase and the separation phase in bean explants.

2 Present address: Department of Horticulture, Oregon State University, Corvallis, OR 97330.

1 Scientific Paper No. SP6528, College of Agriculture Research Center, Washington State University, Pullman, WA; Project 0321.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1984 by the American Society of Plant Biologists