This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lin, W. Articles by Giaquinta, R. T. Search for Related Content PubMed PubMed Citation Articles by Lin, W. Articles by Giaquinta, R. T. Agricola Articles by Lin, W. Articles by Giaquinta, R. T.

Articles

Sugar Transport into Protoplasts Isolated from Developing Soybean Cotyledons ¹

I. Protoplast Isolation and General Characteristics of Sugar Transport

Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898

A procedure is described to isolated functional protoplasts from developing soybean (Glycine max L. Merr. cv Wye) cotyledons. Studies of sucrose and hexose uptake into these protoplasts show that the plasmalemma of cotyledons during the stage of rapid seed growth contains a sucrose-specific carrier which is energetically and kinetically distinct from the system(s) involved in hexose transport. For example, sucrose, but not hexose uptake: (a) is inhibited by alkaline pH and the nonpermeant SH modifier, p-chloromercuribenzene sulfonic acid; (b) is stimulated by fusicoccin; (c) shows both a saturable and a linear component of uptake in response to substrate concentration; and (d) displays a sharp temperature response (high Q₁₀ value and high activation energies).

¹ Contribution No. 3340 from Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, DE 19898. The protoplast isolation procedure and preliminary results of sucrose uptake have been reported at the Annual Meeting of the American Society of Plant Physiologists at Colorado State University, Fort Collins, CO, August, 1983.

This article has been cited by other articles:

				L. A. S. Contim, A. J. Waclawovsky, N. Delu-Filho, C. P. Pirovani, W. R. Clarindo, M. E. Loureiro, C. R. Carvalho, and E. P. B. Fontes The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue-specific expression of the SBP2 promoter J. Exp. Bot., December 1, 2003; 54(393): 2643 - 2653. [Abstract] [Full Text] [PDF]

				E. Peiter and S. Schubert Sugar uptake and proton release by protoplasts from the infected zone of Vicia faba L. nodules: evidence against apoplastic sugar supply of infected cells J. Exp. Bot., July 1, 2003; 54(388): 1691 - 1700. [Abstract] [Full Text] [PDF]

				G. Ritte, J. Rosenfeld, K. Rohrig, and K. Raschke Rates of Sugar Uptake by Guard Cell Protoplasts of Pisum sativum L. Related to the Solute Requirement for Stomatal Opening Plant Physiology, October 1, 1999; 121(2): 647 - 656. [Abstract] [Full Text]

				P. J. Overvoorde, W. S. Chao, and H. D. Grimes A Plasma Membrane Sucrose-binding Protein That Mediates Sucrose Uptake Shares Structural and Sequence Similarity with Seed Storage Proteins but Remains Functionally Distinct J. Biol. Chem., June 20, 1997; 272(25): 15898 - 15904. [Abstract] [Full Text] [PDF]