Plant Physiol.
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Plant Physiology 74:656-662 (1984)
© 1984 American Society of Plant Biologists

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Articles

Spore Germination and Rhizoid Differentiation in Onoclea sensibilis

A Two-Dimensional Electrophoretic Analysis of the Extant Soluble Proteins 1

Clark S. Huckaby and John H. Miller

Department of Biology, Syracuse University, Syracuse New York 13210

Following a geometrically asymmetrical cell division during germination of spores of the fern Onoclea sensibilis L., the small cell differentiates into a rhizoid and the large cell divides to form the protonema. Using silver-staining of two-dimensional gels, we have examined the soluble proteins of spores during germination and of separated rhizoid protoplasts and protonemal cells. Of over 500 polypeptides followed, nearly 25% increased or decreased in prominence during spore germination and the initial phases of rhizoid elongation. Soluble proteins from purified protoplasts of young rhizoids were quantitatively different from those of protonemal cells and germinated spores. Nine polypeptides which appeared after cell division were substantially more prominent in rhizoid protoplasts than in whole germinated spores and have been putatively designated rhizoid-specific polypeptides. The differences in the soluble protein composition of young rhizoids and protonemal cells probably reflect the differential organelle distribution between the two cells as well as differential net protein synthesis in the cytoplasms of the two cells.

1 This research was supported by National Science Foundation Grant PCM-8110079 and by grants from Sigma Xi and the Syracuse University Senate.






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Copyright © 1984 by the American Society of Plant Biologists