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Plant Physiology 74:442-444 (1984)
© 1984 American Society of Plant Biologists

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Articles

Vacuolar Localization of Ethylene-Induced Chitinase in Bean Leaves 1

Thomas Boller and Urs Vögeli

Botanisches Institut, Universität Basel, CH-4056 Basel, Switzerland

The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular chitinase activity.

1 Supported by Swiss National Science Foundation Grant 3.678-080.




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K. BROGUE, I. CHET, M. HOLLIDAY, R. CRESSMAN, P. BIDDLE, S. KNOWLTON, C. J. MAUVAIS, and R. BROGLIE
Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani
Science, November 22, 1991; 254(5035): 1194 - 1197.
[Abstract] [PDF]


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Copyright © 1984 by the American Society of Plant Biologists