Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 74:355-359 (1984)
© 1984 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rebeille, F.
Right arrow Articles by Douce, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rebeille, F.
Right arrow Articles by Douce, R.
Agricola
Right arrow Articles by Rebeille, F.
Right arrow Articles by Douce, R.
Articles

Is the Cytosolic Pi Concentration a Limiting Factor for Plant Cell Respiration?

Fabrice Rebeille, Richard Bligny and Roland Douce

Physiologie Cellulaire Végétale. E.R.A. 847, Centre d'Etudes Nucléaires et Université Scientifique et Médicale de Grenoble-85 X, 38041 Grenoble Cédex, France

The substrate-dependent O2 uptake by sycamore (Acer pseudoplatanus L.) cell mitochondria in the presence of ADP and limiting Pi concentrations has been measured. The Pi concentration for half-maximum O2 uptake rate was found to be in the range 20 to 50 micromolar for all the substrates tested. 31P NMR of intact sycamore cells indicated that the Pi concentration in the cytoplasm was in the range 5 to 6 millimolar, approximately 100-fold higher than the Pi concentration required for maximum O2 uptake rates by isolated mitochondria. When sycamore cells were transferred to a culture medium devoid of Pi, the cytoplasmic Pi concentration decreased from 6 to less than 3 millimolar, but the intact cell respiration remained practically constant for at least 4 days. These results strongly suggest that, in vivo, the respiration rate of sycamore cells is not limited by the quantity of Pi supplied to the mitochondria.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. L. Linster, L. N. Adler, K. Webb, K. C. Christensen, C. Brenner, and S. G. Clarke
A Second GDP-L-galactose Phosphorylase in Arabidopsis en Route to Vitamin C: COVALENT INTERMEDIATE AND SUBSTRATE REQUIREMENTS FOR THE CONSERVED REACTION
J. Biol. Chem., July 4, 2008; 283(27): 18483 - 18492.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Jouhet, E. Marechal, B. Baldan, R. Bligny, J. Joyard, and M. A. Block
Phosphate deprivation induces transfer of DGDG galactolipid from chloroplast to mitochondria
J. Cell Biol., December 6, 2004; 167(5): 863 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Gout, A.-M. Boisson, S. Aubert, R. Douce, and R. Bligny
Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies
Plant Physiology, February 1, 2001; 125(2): 912 - 925.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1984 by the American Society of Plant Biologists