Plant Physiology 74:324-328 (1984)
© 1984 American Society of Plant Biologists
Articles
A Debranching Enzyme Deficiency in Endosperms of the Sugary-1 Mutants of Maize 1
David Pan and
Oliver E. Nelson
Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706
Many of the sugary-1 mutants of maize (Zea mays L.) have the highly branched water-soluble polysaccharide, phytoglycogen, in quantities equal to or greater than starch as an endosperm storage product in mature seeds. We find that all sugary mutants investigated are deficient in debranching enzyme [ -(1, 6)-glucosidase] activity in endosperm tissue 23 days postpollination and suggest that this deficiency is the primary biochemical lesion leading to phytoglycogen accumulation in sugary endosperms. This would indicate that the amylopectin component of starch depends on an equilibrium between the activities of branching enzymes introducing -1,6 branch points into the linear -1,4 glucans and debranching enzymes. The debranching enzyme activities from nonsugary endosperms can be separated into three peaks on a hydroxyapatite column. The sugary endosperm extracts lack one of these peaks of activity while the other two fractions have much reduced activity. The embryos of developing seeds (23 days after pollination) from both sugary and nonsugary genotypes have equivalent debranching activity. The debranching enzyme activity of developing endosperms is proportional to the number of copies (0 to 3) of the nonmutant (Su) allele present suggesting that the Su allele may be the structural gene for this debranching enzyme, although this is not definitive. This identification of debranching enzyme activity as being the biochemical lesion in sugary endosperms is consistent with several previous observations on the mutant.
1 Supported by the College of Agriculture and Life Sciences, University of Wisconsin-Madison and by Department of Energy Contract No. DE-AC02-82ER12031. Paper No. 2670 from the Department of Genetics.
This article has been cited by other articles:
|
|
|
|
 
F. Wattebled, Y. Dong, S. Dumez, D. Delvalle, V. Planchot, P. Berbezy, D. Vyas, P. Colonna, M. Chatterjee, S. Ball, et al.
Mutants of Arabidopsis Lacking a Chloroplastic Isoamylase Accumulate Phytoglycogen and an Abnormal Form of Amylopectin
Plant Physiology,
May 1, 2005;
138(1):
184 - 195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Fujita, A. Kubo, D.-S. Suh, K.-S. Wong, J.-L. Jane, K. Ozawa, F. Takaiwa, Y. Inaba, and Y. Nakamura
Antisense Inhibition of Isoamylase Alters the Structure of Amylopectin and the Physicochemical Properties of Starch in Rice Endosperm
Plant Cell Physiol.,
June 15, 2003;
44(6):
607 - 618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Dinges, C. Colleoni, M. G. James, and A. M. Myers
Mutational Analysis of the Pullulanase-Type Debranching Enzyme of Maize Indicates Multiple Functions in Starch Metabolism
PLANT CELL,
March 1, 2003;
15(3):
666 - 680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hussain, A. Mant, R. Seale, S. Zeeman, E. Hinchliffe, A. Edwards, C. Hylton, S. Bornemann, A. M. Smith, C. Martin, et al.
Three Isoforms of Isoamylase Contribute Different Catalytic Properties for the Debranching of Potato Glucans
PLANT CELL,
January 1, 2003;
15(1):
133 - 149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Dinges, C. Colleoni, A. M. Myers, and M. G. James
Molecular Structure of Three Mutations at the Maize sugary1 Locus and Their Allele-Specific Phenotypic Effects
Plant Physiology,
March 1, 2001;
125(3):
1406 - 1418.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Kubo, N. Fujita, K. Harada, T. Matsuda, H. Satoh, and Y. Nakamura
The Starch-Debranching Enzymes Isoamylase and Pullulanase Are Both Involved in Amylopectin Biosynthesis in Rice Endosperm
Plant Physiology,
October 1, 1999;
121(2):
399 - 410.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. A. Burton, X.-Q. Zhang, M. Hrmova, and G. B. Fincher
A Single Limit Dextrinase Gene Is Expressed Both in the Developing Endosperm and in Germinated Grains of Barley
Plant Physiology,
March 1, 1999;
119(3):
859 - 872.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. K. Beatty, A. Rahman, H. Cao, W. Woodman, M. Lee, A. M. Myers, and M. G. James
Purification and Molecular Genetic Characterization of ZPU1, a Pullulanase-Type Starch-Debranching Enzyme from Maize
Plant Physiology,
January 1, 1999;
119(1):
255 - 266.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Dauvillée, C. Colleoni, E. Shaw, G. Mouille, C. D'Hulst, M. Morell, M. S. Samuel, B. Bouchet, D. J. Gallant, A. Sinskey, et al.
Novel, Starch-Like Polysaccharides Are Synthesized by an Unbound Form of Granule-Bound Starch Synthase in Glycogen-Accumulating Mutants of Chlamydomonas reinhardtii
Plant Physiology,
January 1, 1999;
119(1):
321 - 330.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. C. Zeeman, T. Umemoto, W.-L. Lue, P. Au-Yeung, C. Martin, A. M. Smith, and J. Chen
A Mutant of Arabidopsis Lacking a Chloroplastic Isoamylase Accumulates Both Starch and Phytoglycogen
PLANT CELL,
October 1, 1998;
10(10):
1699 - 1712.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z.-P. Zhu, C. M. Hylton, U. Rössner, and A. M. Smith
Characterization of Starch-Debranching Enzymes in Pea Embryos
Plant Physiology,
October 1, 1998;
118(2):
581 - 590.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Rahman, K.-s. Wong, J.-l. Jane, A. M. Myers, and M. G. James
Characterization of SU1 Isoamylase, a Determinant of Storage Starch Structure in Maize
Plant Physiology,
June 1, 1998;
117(2):
425 - 435.
[Abstract]
[Full Text]
|
|
|
|
|
